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What is bioburden?

  1. The number of microorganisms contaminating an object

  2. The level of cleanliness in the decontamination room

  3. The intensity of ultrasonic cleaning cycles

  4. The presence of detergent residue on instruments

The correct answer is: The number of microorganisms contaminating an object

Bioburden refers to the number of microorganisms present on an object before undergoing the cleaning or sterilization process. It is crucial to accurately determine the initial bioburden as it can affect the effectiveness of subsequent decontamination and sterilization processes. The other options are not correct as they do not accurately describe what bioburden is.